Influence of follicular maturation on 3-hydroxy-methylglutaryl coenzyme A reductase activity in hen granulosa cells

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Authors:
E. K. Asem
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and
F. Hertelendy
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DOI:
https://doi.org/10.1530/jrf.0.0790215
Volume/Issue:
Volume 79: Issue 1
Page Range:
215–221
Article Type:
Research Article
Online Publication Date:
Jan 1987
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Summary. The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase: EC 1.1.1.34) was measured in a microsomal preparation of the granulosa of rapidly growing ovarian follicles of laying hens in the late preovulatory period (2–3 h before expected ovulation). The specific activity of the enzyme was measured in the five largest (F1–F5) preovulatory follicles, F1 being the follicle destined to ovulate first. Enzyme activity increased concomitantly with follicle size. The apparent Km of the enzyme decreased 60–80% from the smallest to the largest preovulatory follicle. There was no significant change in the Vmax during follicle development. Although our results have demonstrated the presence of HMG/CoA reductase in chicken granulosa cells and the progressive increase of its activity with follicular maturation, the quantitative significance of de-novo synthesized cholesterol as steroid hormone precursor remains to be ascertained.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899