The effects of cryopreservation and transfer on embryonic development in the common marmoset monkey, Callithrix jacchus

in Reproduction
Authors:
P. M. Summers
Search for other papers by P. M. Summers in
Current site
Google Scholar
PubMed Close
,
A. M. Shephard
Search for other papers by A. M. Shephard in
Current site
Google Scholar
PubMed Close
,
C. T. Taylor
Search for other papers by C. T. Taylor in
Current site
Google Scholar
PubMed Close
, and
J. P. Hearn
Search for other papers by J. P. Hearn in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1530/jrf.0.0790241
Volume/Issue:
Volume 79: Issue 1
Page Range:
241–250
Article Type:
Research Article
Online Publication Date:
Jan 1987
Free access

Sign up for journal news

Summary. Embryos were collected at the 4–10-cell stage from the oviducts (Day 4; Day 1 = ovulation) or as morulae (Day 7) from the uterus of marmosets and frozen in 1·5 m-DMSO (Days 4 and 7) or 1·0 m-glycerol (Day 4 only), using a slow freezing and thawing technique. Of 22 Day-4 embryos frozen in DMSO, 18 were recovered and 16 of these were transferred to 10 synchronized recipients; 7 recipients became pregnant compared with all 7 control recipients receiving 10 unfrozen embryos. Fifteen frozen– thawed morulae were transferred to 9 Day-6 recipients; the pregnancy rate (55·6%) was lower than for control embryos (85·7%). Embryos frozen in glycerol suffered severe osmotic stress during glycerol addition and removal. Of 8 recipients, 3 (37·5%) became pregnant but only one fetus was carried to term.

These results on embryo collection, freezing and transfer in the marmoset have important implications for developing improved methods for freezing human embryos and the breeding of endangered primates.

Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 197 47 2
PDF Downloads 147 58 1

 

  • Collapse
  • Expand
Print ISSN:
1470-1626
Online ISSN:
1741-7899