Effects of oestradiol and progesterone on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro

in Reproduction
Authors:
N. K. Khurana
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and
R. G. Wales
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DOI:
https://doi.org/10.1530/jrf.0.0790267
Volume/Issue:
Volume 79: Issue 1
Page Range:
267–273
Article Type:
Research Article
Online Publication Date:
Jan 1987
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Summary. The addition of progesterone (10−7 to 10−5 m) and/or oestradiol (10−10 m) during 24-h chase culture of pulse-labelled morulae–early blastocysts did not affect the degradation of radiolabelled glycogen or other biochemical fractions. The presence of a high concentration of progesterone (10−5m) during 5-h pulse culture significantly inhibited incorporation of substrate carbon from [U-14C]glucose into both the acid-soluble and acid-insoluble glycogen fractions, but had no effect on non-glycogen fractions. Catabolic utilization of glucose as estimated by the rate of carbon dioxide and lactate production was not affected by the presence of progesterone (10−7 to 10− 5m), oestradiol (10−10 to 10−8m) or a combination of both. The results indicate that ovarian steroids at expected physiological concentrations do not directly influence embryonic energy metabolism.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899