Effects of antibiotics and medium supplements on steroidogenesis in cultured cow luteal cells
in ReproductionSearch for other papers by J. P. Poff in
Current site
Google Scholar
PubMed
Search for other papers by D.L. Fairchild in
Current site
Google Scholar
PubMed
Search for other papers by W. A. Condon in
Current site
Google Scholar
PubMed
Summary. Corpora lutea were removed from regularly cycling dairy cows, dissociated with collagenase and cultured for 8 or 10 days in Ham's F-12 medium. In Exp. 1 treatment with insulin, or an insulin–transferrin–selenium combination (ITS), increased progesterone production from basal levels on Day 4 of culture to 234% (P < 0·01) above controls on Day 10. LH alone increased progesterone production 45% above controls on Day 10 (P > 0·05). When LH was combined with insulin or ITS, progesterone production was stimulated to an average of 1802% (P < 0·01) above controls on Day 10 of culture. Transferrin or selenium without insulin did not allow LH to stimulate progesterone synthesis. In Exp. II, LH alone or LH plus gentamicin or penicillin–streptomycin increased progesterone production from basal levels on Day 2 steadily to an average of 468% (P < 0·01) above controls (no antibiotics) by Day 8 of culture. The addition of amphotericin-B, alone or in combination with the other antibiotics, inhibited all LH-stimulated progesterone synthesis, but did not affect basal progesterone levels. We conclude that insulin is essential for maximal steroidogenesis in a bovine luteal cell culture system, and that LH-stimulated progesterone production is inhibited in the presence of amphotericin-B, but is not inhibited by gentamicin or penicillin–streptomycin. The elimination of amphotericin-B, coupled with the addition of insulin to the cell culture system increased the responsiveness of the cells to LH. These culture conditions represent the first report in which LH increased total progesterone production for 10 days, maintaining luteal function in a chemically-defined culture system.
Keywords: antibiotics; luteal cells; progesterone synthesis
Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 192 | 52 | 1 |
| PDF Downloads | 105 | 34 | 4 |
Online ISSN: 1741-7899
Print ISSN: 1470-1626
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519