Mechanism of action of (−)gossypol on ATP production in isolated hamster spermatids

in Reproduction
Authors:
P. J. Den Boer
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J. A. Grootegoed
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DOI:
https://doi.org/10.1530/jrf.0.0830693
Volume/Issue:
Volume 83: Issue 2
Page Range:
693–700
Article Type:
Research Article
Online Publication Date:
Jul 1988
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Summary. The ATP content of round spermatids isolated from hamsters was decreased 90% after 18 h of incubation in the presence of 4μm-(−)gossypol and 0·10% bovine serum albumin (BSA). The (+)-enantiomer had no effect under these incubation conditions. The Michaelis–Menten constant Km and the maximal initial velocity Vmax of cellular LDH-C4 were not significantly altered after 18 h of incubation of the spermatids with (−)gossypol. Furthermore, there was no effect of (−)gossypol on the production of 14CO2 from l-[U-14C]lactate. It is concluded that (−)gossypol does not inhibit ATP production in spermatids by an effect on the sperm-specific LDH-C4 enzyme or on the mitochondrial oxidation of pyruvate. Rather, (−)gossypol may have an effect on the coupling between electron transport and ATP synthesis in the mitochondria. This action of (−)gossypol may not involve the H+-conducting activity of gossypol, but could be produced through binding of (−)gossypol to specific mitochondrial proteins.

Keywords: spermatids; gossypol; ATP; LDH-C4; mitochondria; enantiomers

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Print ISSN:
1470-1626
Online ISSN:
1741-7899