Differential effects of (+)- and (−)-gossypol enantiomers on LDH-C4 activity of hamster spermatogenic epithelium in vitro

in Reproduction
Authors:
P. J. Den Boer
Search for other papers by P. J. Den Boer in
Current site
Google Scholar
PubMed Close
and
J. A. Grootegoed
Search for other papers by J. A. Grootegoed in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1530/jrf.0.0830701
Volume/Issue:
Volume 83: Issue 2
Page Range:
701–709
Article Type:
Research Article
Online Publication Date:
Jul 1988
Free access

Sign up for journal news

Summary. Tubular fragments (spermatogenic epithelium) from immature hamsters were isolated and cultured with low doses of (+)- and (−)-gossypol enantiomers. The activity of lactate dehydrogenase isoenzyme LDH-C4 was estimated as a marker for spermatogenic cell development and α-ketoisovalerate was used as the substrate. In the absence of gossypol, the specific activity of LDH-C4 in the tubular fragments was increased 40% during incubation for 48 h. This developmental increase was suppressed by gossypol. The specific activity of LDH-C4 in the tubular fragments was lowered by gossypol, after 48 h of culture in the presence of low doses of racemic gossypol (1–4 μm) and 1% fetal calf serum. In this in-vitro system the (−)-enantiomer but not the (+)-enantiomer of gossypol affected the LDH-C4 activity. This is in agreement with other reports showing that only the (−)-enantiomer induces infertility. The observed action of gossypol on LDH-C4 activity in the tubular fragments may reflect gossypol-induced degeneration of spermatogenic cells. The present in-vitro system can be used to estimate the actions of gossypol derivatives, other investigational antifertility agents, and toxic agents on the spermatogenic epithelium.

Keywords: gossypol enantiomers; spermatogenic epithelium; LDH-C4; hamster

Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 178 54 16
PDF Downloads 52 21 2

 

  • Collapse
  • Expand
Print ISSN:
1470-1626
Online ISSN:
1741-7899