in Reproduction
In a recent communication (Wales & Wallace, 1964), it was observed that the oxidation of substrates other than fructose was greatly depressed by washing the spermatozoa free of their seminal plasma. This effect could be due either to the removal of protective macromolecules in the seminal plasma or to the removal of alternative substrates. The following experiments were undertaken to investigate these possibilities.Veronal-buffered saline (pH 7·2) was the basic diluent used and the methods for preparing washed suspensions, for incubating the spermatozoa, for the assay of radioactivity and for the estimation of lactate and fructose, have been previously described (Wallace & Wales, 1964; Wales & Wallace, 1964). Sorbitol was estimated by an enzymic method (T. O'Shea and R. G. Wales, unpublished data). Acetate was estimated after steam distillation of metaphosphoric acid extracts (Annison, 1954). The

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