Effect of prostaglandins on luteal function during early pregnancy in pigs

in Reproduction
Authors:
T. Wiesak
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M. G. Hunter
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, and
G. R. Foxcroft
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DOI:
https://doi.org/10.1530/jrf.0.0950831
Volume/Issue:
Volume 95: Issue 3
Page Range:
831–840
Article Type:
Research Article
Online Publication Date:
Aug 1992
Free access

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Summary. Luteal cells were obtained by digestion of luteal tissue of cyclic (day 12) and early pregnant (days 12, 20 and 30) pigs. Suspensions of the dispersed luteal cells (5 × 104 cells ml−1) were incubated for 2 h in minimum essential medium (MEM) alone (control) and MEM with different concentrations of prostaglandin F2α (PGF2α) and PGE2 (0·01, 0·1, 1, 10, 100 and 1000 ng ml−1) and luteinizing hormone (LH) 100 and 1000 ng ml−1, or with combinations of LH + PGF2αand LH + PGE2. Net progesterone production was measured in the incubation media by direct radioimmunoassay.

The overall response pattern of the luteal cells to exogenous hormones on day 12 of the oestrous cycle and pregnancy differed (P < 0·5) from treatment on day 20 and 30 of pregnancy. In general progesterone production was higher (P < 0·5) and the response to PGF2α and PGE2 treatment was most obvious on day 12 of the oestrous cycle and pregnancy. Overall, PGF2α stimulated progesterone production in a dosedependent manner (P < 0·05). The response to PGE2 was of a quadratic nature (P < 0·05) in which the lowest and the highest doses of PGE2 were associated with a greater production of progesterone than were the intermediate doses. Treatment of luteal cells with PGF2α + LH or PGE2 + LH caused overall inhibition (P < 0·05) of progesterone production compared with treatment with each hormone alone. This interaction was not affected by the dose of LH used.

These findings indicate that PGF2α and PGE2 are involved in the autocrine control of corpus luteum function.

Keywords: pig; luteal cells; prostaglandins; pregnancy

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Print ISSN:
1470-1626
Online ISSN:
1741-7899