In vitro fertilization and embryo development in the marmoset monkey (Callithrix jacchus)
in ReproductionSearch for other papers by L. J. Wilton in
Current site
Google Scholar
PubMed
Search for other papers by V. S. Marshall in
Current site
Google Scholar
PubMed
Search for other papers by E. C. Piercy in
Current site
Google Scholar
PubMed
Search for other papers by H. D. M. Moore in
Current site
Google Scholar
PubMed
Oocytes aspirated from preovulatory (i.e. ≥ 2 mm) follicles of marmoset monkeys were graded for maturity according to the degree of cumulus expansion, grade I being most mature and grade IV least mature. After preincubation for 2–5, 9–11 or 21–29 h, 82% of oocytes could be fertilized using epididymal spermatozoa and only 2.3% were polyspermic. Fertilization rate was lowest (60%) in grade IV oocytes and all oocytes preincubated for 2–5 h (53%). Fertilization rate increased to 92% in oocytes preincubated for 21–29 h. Embryos developed in vitro to a mean of eight cells. Embryo development was unaffected by oocyte maturity but correlated with preincubation time. Oocytes preincubated for 2–5 h developed into embryos with significantly fewer cells than those preincubated for 9–11 or 21–29 h (P < 0.001). Fifty-six per cent of embryos showed delayed cleavage and these had fewer cells than non-delayed embryos (P < 0.001). When oocytes were preincubated for 2–5 h, development of all resulting embryos was delayed. However, only 17 and 58% of embryos developing from oocytes preincubated for 9–11 and 21–29 h, respectively, were delayed and this was independent of oocyte maturity.
Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 166 | 44 | 1 |
| PDF Downloads | 142 | 42 | 3 |
Online ISSN: 1741-7899
Print ISSN: 1470-1626
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519