Comparison of different antibody–conjugate derivatives for the development of a sensitive and specific progesterone assay
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Antibodies raised against progesterone with hormone-carrier protein bridges placed at four different carbon positions were used to compare the sensitivity and specificity of homologous and heterologous enzyme–hormone conjugates. All heterologous assays were at least twice as sensitive as the corresponding homologous assays. The best results were obtained by using antibodies against 7α-carboxyethyl-thioether-progesterone with 6β-hemisuccinate–progesterone conjugate (or hemimaleate). The sensitivity with human sera was 0.25 ng ml−1 and, the highest crossreaction 10% with 5β-pregnane-3,20-dione, and reproducibility, recovery and accuracy were satisfactory. The correlation coefficient with radioimmunoassay in 103 human sera tested was r = 0.915. The assay was successfully applied for the diagnosis of pregnancy in dairy cattle.
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